TonB-dependent transporters and their occurrence in cyanobacteria

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Signal transduction pathway of TonB-dependent transporters.

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Gating of TonB-dependent transporters by substrate-specific forced remodelling

Membrane proteins play vital roles in inside-out and outside-in signal transduction by responding to inputs that include mechanical stimuli. Mechanical gating may be mediated by the membrane or by protein(s) but evidence for the latter is scarce. Here we use force spectroscopy, protein engineering and bacterial growth assays to investigate the effects of force on complexes formed between TonB a...

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Substrate-dependent transmembrane signaling in TonB-dependent transporters is not conserved.

Site-directed spin labeling (SDSL) was used to examine and compare transmembrane signaling events in the bacterial outer-membrane transport proteins BtuB, FecA, and FhuA. These proteins extract energy for transport by coupling to the transperiplasmic protein TonB, an interaction that is thought to be mediated by the Ton box, a highly conserved energy-coupling motif in these transporters. In the...

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Reconstitution of bacterial outer membrane TonB-dependent transporters in planar lipid bilayer membranes.

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ژورنال

عنوان ژورنال: BMC Biology

سال: 2009

ISSN: 1741-7007

DOI: 10.1186/1741-7007-7-68